FastANI tool: to compare draft and reference genomes
High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries.
Jain, C., Rodriguez-R, L.M., Phillippy, A.M. et al., Nature Communication (2018).
https://github.com/ParBLiSS/FastANI
fastANI -h
-r <value>, --ref <value>
reference genome (fasta/fastq)[.gz]
-q <value>, --query <value>
query genome (fasta/fastq)[.gz]
°°°
fastANI -q draft_genome.fasta -r reference_genome.fasta -o fastani.out
cat fastani.out
draft_genome.fasta reference_genome.fasta 96.41 330 352
Mapping result
Nucleotide Identity (ANI) = 96.41
330 of total 352 sequence fragments were aligned as orthologous matches