Quality Control & Normalization


→ Coverage depth

How many short reads mapped to a genome sequence?

→ Sample size

How deep do we have to sequence?

→ RPKM calculation

Normalization for comparing gene coverage values

→ Phred score (Q score)

Measure for base quality

→ Giga base pairs

Sequence length in numbers of base pairs

→ GC content

Guanine-cytosine (GC) content of a genome sequence