RNA-seq versus microarray

Metatranscriptomics (RNA-seq)

Microarrays have achieved its technological limits and hence in many studies mircoarrays are increasingly replaced by RNA sequencing.


  • does not require a priori knowledge of the RNA sequence

      • discover novel transcripts (gene finding)

      • detect single nucleotide polymorphisms (SNPs)

  • increasing accuracy

      • provides absolute transcript levels (more precise than hybridization)

      • no cross-hybridization issues

  • larger range from small to high expression levels

      • better accuracy in very small and very large values

But RNA-seq is still more expensive than microarray technology and requires higher computational resources for storing and analyzing the data.

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