Aligning and merging short fragments of sequenced DNA in order to reconstruct the original genome.
mapping reads to reference genomes
de novo assembly approach
assembling reads without help of a similar reference sequence
→ N50 statistics
short reads assembler
microbial community assembly (metagenomics)
MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph
Scaffolding pre-assembled contigs using SSPACE
SSPACE-LongRead: scaffolding bacterial draft genomes using long read sequence information.
Fast assembler for small genomes
Designed for assembling large plant and animal genomes
Comparison and algorithmic background of assemblers