Genome quality & annotation

Metagenomics Tools

Genome download

→ NCBI refseq and genbank


Genome quality control

→ CheckM

to estimate genome com­pleteness and contamination based on common single-​copy genes


Genome annotation

→ Prokka

Annotation tool for bacterial, archaeal, and viral genomes

→ RAST server

for bacterial and archaeal genomes

→ Maker

eukaryotic and prokaryotic genomes

→ GeneMark

- eukaryotics; prokaryotics; viruses, pages and plasmids

- part of genome annotation pipelines at NCBI

→ MetaGeneMark

gene identification in metagenomic sequences


Pathway / gene / protein databases

→ KEGG

gene annotation and pathway database

→ KEGG KAAS server

multiple sequence annotation

→ WikiPathways

Community edited pathway database

→ UniProt

Gene annotation and protein database

→ Pfam

Gene annotation and protein database


Converting gene/protein/pathways identifiers

convert UniProt, KEGG, EMBL, RefSeq, ... identifiers

http://www.uniprot.org/uploadlists/

UniProf or NCBI-geneIDs to KEGG

http://www.genome.jp/kegg/tool/conv_id.html