Error while running "split_libraries_fastq.py"

split_libraries_fastq.py -i JoinedReads/SampleA_L001_R1_001/fastqjoin.join.fastq,JoinedReads/SampleB_L001_R1_001/fastqjoin.join.fastq, --sample_ids SampleA,SampleB -o SEQ/ -q 19 --barcode_type 'not-barcoded'

Error in split_libraries_fastq.py: No filepaths match pattern/name ''. All patterns must be matched at least once.


a) Typical (copy/paste) error is an additional comma behind the last sample-filename

... fastq, --sample_ids ...

b) Or, frequent mistake is also a space behind comma

...A_L001_R1_001/fastqjoin.join.fastq, B_L001_R1_001/fastqjoin.join.fastq ...


a) Use comma only between samples, not behind the last sample

... fastq --sample_ids ...

b) Don't uses spaces in between the sample-filenames

...A_L001_R1_001/fastqjoin.join.fastq,B_L001_R1_001/fastqjoin.join.fastq ...

Error in split_libraries_fastq.py: Positional argument detected

Also, don't use spaces beween sampleID's

... SampleA,SampleB ...