Tools‎ > ‎Shotgun sequencing‎ > ‎NCBI SRA file format‎ > ‎

wget download

Better use prefetch or fastq-dump download, instead of  wget !

Many .sra files are compressed against a reference which is not included in the .sra file.

The SRA-tools prefetch or fastq-dump automatically download both the .sra files and the reference sequences required for decompressing. Additionally it gives download success control.

Download using wget

(not recommended)

# get SRA file location on NCBI server (alternatively, search at NCBI website)
srapath SRR649944

# download using wget
wget -P Data

ls Data/

# converting SRA file into fastq
fastq-dump --split-3 Data/SRR649944



many samples will not work using wget, as the compression related reference genome is missing
Solution: use prefetch or fastq-dump, instead of wget

fastq-dump --split-3 SRR649944 (only access number without path  Data/SRR649944 or ./SRR649944) will force fastq-dump to download the sample directly from NCBI instead of using the local copy.