for mapping a nucleotide sequence against a protein database BLASTx optionsnucleotide sequences are translated automatically by BLAST blastx -query DNA_gene_sequences.ffn -subject protein_sequences.faa -outfmt 6 -evalue 1e-50http://www.ncbi.nlm.nih.gov/books/NBK279675/table/appendices.T.blastx_application_options/ |