Calculate how deep sequencing for a required genome coverage. genomeLength x coverage x 100 / abundance = samplingDepth genomeLength - bacterial genome lengthcoverage - required coverageabundance - expected relative abundance level (percent)Genome length: 5Mbp Required coverage: 2X expected abundance level: 0.5 % 5Mbp x 2 x 100 / 0.5 = 2Gbp 5,000,000 x 2 x 100 / 0.5 = 2,000,000,000 A sampling size of 2Gbp is required to get a 2X coverage of a 5Mbp genome present at 0.5% abundance level How many reads? (for example: 200bp read length) samplingDepth / readLength 2,000,000,000 bp / 200 bp = 10,000,000 reads |