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RNA-seq versus microarray

Microarrays have achieved its technological limits and hence in many studies mircoarrays are increasingly replaced by RNA sequencing.


  • does not require a priori knowledge of the RNA sequence
    • discover novel transcripts (gene finding)
    • detect single nucleotide polymorphisms (SNPs)
  • increasing accuracy
    • provides absolute transcript levels (more precise than hybridization)
    • no cross-hybridization issues
  • larger range from small to high expression levels
    • better accuracy in very small and very large values

But RNA-seq is still more expensive than microarray technology and requires higher computational resources for storing and analyzing the data.